An Introduction to General Virology by Thomas M. Bell (Auth.)

By Thomas M. Bell (Auth.)

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Pub. Health Assoc. Salk, J. , Youngner, J. , Ward, E. N. (1954) Amer. J. Hyg. 60, 214. Lipton, M. , Steigman, A. J. (1955) Proc. Soc. Exp. Biol. Med. 88,114. Johnston, P. , Grayston, J. , Loosli, C. G. (1957) Proc. Soc. Exp. Biol. Med. 94, 338. , Coleman, V. R. (1952) /. Immunol. 68, 645. , Strickland, A. G. R. (1956) Virology, 2, 162. Porterfield, J. S. (1960) Bull. Wld. Hlth. Org. 22, 373. Daniels, J. , Ratner, J. , Brown, S. R. (1961) Science, 133, 640. SEROLOGY 17. 18. 19. 20. 21. 22. 23. 24.

These mixtures are incubated for 1-6 hours at room temperature or 37°C depending on the virus. Then 1 ml is inoculated into tissue culture tubes which have been drained of medium. A control virus titration is also performed and the end point is read when the virus controls have reached their maximum SEROLOGY 39 expected titre. , titre = 90). In laboratories where hundreds of sera are tested monthly a simple and rapid test is now displacing the above method. 10»11'12 The preparation of virus/serum mixtures is identical with the previous test but after incubation, a suspension of susceptible cells is added.

Exp. Biol. Med. 84, 570. 17. Bell, T. , Steyn, J. H. (1962) Brit. Med. J. 2, 700. 18. Farrell, L. , Franklin, A. , Shimada, F. , MacMorine, H. , Rhodes, A. J. (1953) Can. J. Pub. Health, 44, 273. 19. , Vogt, H. (1954) / . Exper. Med. 99, 167. 20. Earle, W. R. (1943) / . Nat. Cancer Inst. 4, 165. 21. Eagle, H. (1955) Proc. Soc. Exp. Biol. Med. 89, 362. 22. Moore, A. , Toolan, H. W. (1955) Cancer Res. 15, 598. 23. Frisch, A. , Losli, E. J. (1955) Amer. J. Clin. Path. IS, 1107. 24. Fogh, J. , Lund, R.

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