An Atlas of Induced Sputum: An Aid for Research and by Ratko. Djukanovic, Peter Sterk

By Ratko. Djukanovic, Peter Sterk

Containing greater than a hundred and fifty photographs of the very best quality, An Atlas of caused Sputum: An reduction for examine and analysis is the 1st complete scientific consultant at the software of this diagnostic strategy to non-infectious inflammatory ailments. The booklet contains contributions from best specialists during this swiftly becoming box who have been contributors within the activity strength on prompted sputum subsidized by way of the ecu breathing Society and chaired by way of the editors of this Atlas, Drs. Djukanovic and Sterk. providing a number of graphs and illustrations, the atlas expertly describes ideas and approaches and gives transparent thoughts for sputum induction and research in keeping with the eu respiration Society job strength document. A diagnostic reference for pulmonologists, the atlas does double responsibility as an instructive textual content for citizens and physicians in education.

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Extra info for An Atlas of Induced Sputum: An Aid for Research and Diagnosis (Encyclopedia of Visual Medicine Series)

Sample text

A minimum of 400 nonsquamous cells is counted to determine the proportion of macrophages, neutrophils, eosinophils, lymphocytes and bronchial epithelial cells. 5). QUALITY CONTROL Every laboratory requires an adequate quality control program, which forms an integral component of standard operating procedure (SOP) protocols. This is particularly important when slide readings are used to monitor patient treatment. Incorrect results can lead to incorrect diagnosis, treatment and research direction.

9 Concentrations of eosinophil cationic proteins (ECP) in supernatants from the selected, viscous portion of sputum and the non-viscous portions of the same samples that remained after selection (residual portion). 001, groups comparison). Adapted with permission from Pizzichini E, Pizzichini MM, Efthimiadis A, et al. Indices of airway inflammation in induced sputum: reproducibility and validity of cell and fluidphase measurements. 10); hence contamination of sputum by saliva probably does not contribute significantly to the levels of these mediators when the Analysis of soluble mediators in the sputum fluid phase 51 whole expectorate is analyzed.

07 mM. Reproduced with permission from Kelly MM, Wightman PD, Hargreave FE, et al. Repeatabllity of leukotriene measurements in induced sputum. 16 The effect of the addition of dithiothreitol (DTT) and/or protease inhibitor (Pl) to standards for nerve growth factor (NGF). The curve is shifted to the right, resulting in a false elevation of measured levels of assayed samples. The addition of Pl has no effect on the standard curve. Reproduced with permission from Kelly MM, Davis P, Leigh R, et al.

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